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After three washes with PB supplemented with 0.3% Triton X-100 (PBT), the sections were incubated overnight at 4C in PBT containing 0.3% BSA (Santa Cruz), 2% normal horse serum (Vector Laboratories), and the mouse anti-NeuN and rabbit anti-TrkA or anti-NGF primary antibodies (at a dilution of 1:200 in all cases except for NeuN, which was used at a dilution of 1:600)

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Additionally, GLUT1 mRNA expression is upregulated by Src or Ras protein, mostly in the presence of two enhancer elements in the gene [10]

Conversely, a lack of SCFA-producing Roseburia and Faecalibacterium (often reduced in PD) means fewer Tregs to keep inflammation in check [163]