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Medications for diabetes and weight loss included semaglutide (sold under the brand names Ozempic and Rybelsus for diabetes, and Wegovy for weight loss), dulaglutide (sold as Trulicity), liraglutide (Victoza and Saxenda, among others), empagliflozin (Jardiance, among others), and metformin (Glumetza, among others)

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2.13 ACE and ACE2 activities To assess angiotensin-converting enzyme (ACE) and ACE2 activity measurement, kidney protein extracts were obtained from kidney cortex homogenized with lysis buffer (50 mM HEPES pH7.4, 150 mM NaCl, 0.5% Triton-X-100, 0.025 mM ZnCl 2 , 0.1 mM Pefabloc SC Plus (11873601001, Roche) and a 1:7 dilution of EDTA-free protease inhibitor cocktail (11836170001, Roche))