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Before drug quantitation, tissues were homogenized at 200 mg ml 1 in a Precellys Evolution homogenizer (Bertin Technologies) in a lysis buffer consisting of 50 mM Tris-HCl, 100 nM NaCl, 0.1% Triton X-100 pH 7.4 with 1 protease inhibitor (Roche A32955) for every 10 ml of buffer at 6,800 rpm for three 20 s cycles with 30 s pauses between cycles

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The molecular mechanisms by which oxidative stress induces sperm damage are diverse and have profound implications for male fertility

To block the c-Myc pathway, a peptide derived from helix 1 (H1) of the HLH region of c-Myc [43] was linked with a CPP and thermoresponsive elastin-like polypeptide (ELP) [108]