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The beads were washed three times with 1 ml of high-salt buffer (20 mM HEPES-KOH (pH 7.9), 500 mM KCl, 0.2 % NP-40, 15 % glycerol, 1 mM DTT) with rotation for 5 min at 4 C during each wash, followed by one detergent-free wash (20 mM HEPES-KOH (pH 7.9), 150 mM KCl, 15% glycerol, 1 mM DTT)

Class : 20,728 particles) were manually grouped, and initial 3D volumes were generated using asymmetric helical reconstruction

Quick reference for 5 mg/mL concentration (2 mL water in 10 mg vial) If you followed the recommended 2 mL reconstitution, every calculation becomes simple: 1 mg dose = 20 units (0.2 mL) 2 mg dose = 40 units (0.4 mL) 2.5 mg dose = 50 units (0.5 mL) 3 mg dose = 60 units (0.6 mL) 4 mg dose = 80 units (0.8 mL) 5 mg dose = 100 units (1.0 mL) See the pattern

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