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Lipolysis assay in brown and white adipocytes and WAT explants Differentiated BA were treated for 4 h with or without 500 nM G49 in Krebs-Ringer Modified Buffer (KRB) (1118.5 mM NaCl, 4.75 mM KCl, 1.92 mM CaCl 2 , 1.19 mM KH 4 PO 4 , 1.19 mM MgSO 4 (H 2 O) 7 , 25 mM NaHCO 3 , 10 mM HEPES, 6 mM D-Glucose, 4% BSA pH 7.4)