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The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the primary antibody (EM1901-56) at 1/2,000 dilution for 1 hour at room temperature

Their contributions have significantly enhanced the quality of our findings

Reduced autophagic flux was also reflected by the partial accumulation of the autophagy adaptor and substrate p62, taken as a surrogate for autophagic flux, in TBC1D9B-depleted cells (Supplementary Fig