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The low clearance of long-chain acylcarnitine molecules leads to a high acylcarnitine-to-L-carnitine ratio that has been associated with a higher risk of cardiovascular mortality (72)

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Some researchers used TALEN technology to simultaneously knock out the FAA1 and FAA4 genes in Saccharomyces cerevisiae , blocking the recycling and utilization of free fatty acids and significantly increasing the accumulation of intracellular fatty acids in yeast [112]