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A neural circuit for the suppression of pain by a competing need state

Dried metabolite extracts were resuspended in 100 L of LCMS-graded water following microcentrifugation for 5 minutes at 21,000 xg at 4 C to pellet any remaining insoluble debris

These results were obtained because of the length of the C-H alkyl chains 58 , low hydrophilic lipophilic balance (HLB) value and the hydrophobic moiety 59 , the higher the gel-liquid phase transition temperature and low surface free energy 57 of Span 60