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In contrast, LPS-induced Raw 264.7 cells (PBS) display significantly reduced fluorescence, suggesting that LPS has impaired the mitochondrial membrane potential of Raw 264.7 cells

At present, the lack of large-scale, confirmatory clinical trials specifically targeting MC4R deficiency is largely due to significant scientific and clinical heterogeneity, which has made conducting such trials logistically challenging and commercially less appealing compared to pursuing broader or more clearly defined patient populations

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