CYP1A1 metabolic activity has also been shown to be increased by approximately 66%70% in smokers, mainly due to exposure to aryl hydrocarbons such as benzo [a]pyrene (Vistisen, Loft, and Poulsen, 1991)
Recently, many researchers have emphasized the connection between the brain and gut, called the gutbrain axis, for treating both CNS neuropathologies and gastrointestinal diseases
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Brains were later sectioned on Leica CM3000 cryostat, and 1 mm punches (Integra Miltex Biopsy Punch) of the hypothalamus, hippocampus and hindbrain were collected