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A linear mixed model (LMM) was employed: where is the trait value for individual , is the intercept, is the effect of genetic locus on individual , and are fixed effect covariates, is a random term representing the polygenic effect (using a leave-one-chromosome-out (LOCO) kinship matrix), and is the error term

Insert the needle through the rubber stopper at an angle, directing the stream of liquid against the glass wall of the vial rather than directly onto the powder cake

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& Sawalha, A