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Educational commentary

Cells Vero African green monkey kidney cells were cultured at 37C in a 5% CO2 atmosphere in Eagles Minimum Essential Medium (MEM, HyClone) containing 1.2 mg/ml NaHCO3 and supplemented with 6% (v/v) foetal bovine serum, 2 mM glutamine, 100 IU/ml penicillin and 100 g/ml streptomycin

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Furthermore, we focus on the role of protein glutathionylation recently found in fused-in-sarcoma (FUS)-associated ALS and discuss whether this glutathionylation has a pathological role in the development and progression of ALS by modulating LLPS of RBPs, particularly FUS