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For analysis of the effect of cell culture conditions, hES-MSCs were seeded at densities of 5 10 3 and 2 10 3 cells/cm 2 , incubated for 19 h, stained with FreSHtracer, and the fluorescence ratio (F 510 /F 580 ) was measured as described above
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The relevant therapeutic agents are summarized in Table A1
Biological Activities Antioxidant activity Glutathione Low cellular concentrations of glutathione, a major intracellular antioxidant, and/or overproduction of reactive oxygen species (ROS) can lead to oxidative stress-induced damage to biological macromolecules and contribute to the development and progression of pathological conditions
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