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Vector construction For constructing pCSII-CAG-Venus-WPRE, a CAG fragment was amplified via PCR and ligated into pCSII-EF-Venus-WPRE [gifted by Dr Miyoshi and Dr Miyawaki (RIKEN, Japan) (Nagai et al., 2002)]

In terms of preventing adverse outcomes, periconceptional folic acid supplementation has a proven role, established more than 30 years ago, in protecting against neural tube defect-affected pregnancies and this has been the major focus of public health policy worldwide

[DOI] [PMC free article] [PubMed] [Google Scholar] 9.Baltazar-Martins G, Brito de Souza D, Aguilar-Navarro M, Munoz-Guerra J, MDM P, Del Coso J