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(A) Quantification of mitochondrial cristae volume, defined as the ratio of the total cristae surface area of each mitochondrion to the mitochondrial area, of 5 mitochondria per cell for a total of 10 cells from each sample (total of 50 mitochondria per cell line
The expression level of ferritin is relatively low in SN dopaminergic neurons, while NM serves as their primary iron storage system
Cellular extracts were added in a final volume of 1.0 ml, to 150 mM Tris HCl buffer, pH 8.2, containing 25 mM L-glutamate, 25 mM L--aminobutyrate, 10 mM ATP, 10 mM phosphoenolpyruvate, 30 mM MgCl 2 , 100 mM KCl, 0.2 mM EDTA, 0.42 mM NADH, 7 IU pyruvate kinase, 12 IU lactate dehydrogenase, and the oxidation of NADH was monitored at 340 nm