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Targeted sequencing of the TP53 SNP DNA of white blood cells was isolated by using the DNeasy Blood and Tissue kit (Thermo Fisher Scientific, Waltham, MA, United States) according to manufacturers instructions

Allow vial to reach room temperature before preparation Draw 2ml bacteriostatic water using a sterile syringe Inject slowly down the inside wall of the vial Avoid foaming Gently swirl until fully dissolved Do not shake vigorously Label with the reconstitution date Refrigerate at 2C8C Protect from direct light exposure Protocol Overview Goal: Support Testicular Function, Fertility & Hormonal Balance Research Route: Subcutaneous Schedule: 3 Times Weekly Dose Range: 500 IU 2,000 IU Per Injection Reconstitution: 2ml BAC Water Final Concentration: 2,500 IU/ml Storage: Refrigerated After Reconstitution Reference Parameters (Standard-Dose protocol) For laboratory research only

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