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Half of the samples were also incubated in 4 l of 10 mM TCEP (Sigma-Aldrich) at room temperature for 30 min, to reduce oxidized thiols

The 2.0 mL dilution gives a clean 5 mg/mL concentration, so a 5 mg dose reads at exactly 100 units (1.0 mL) on a U-100 syringe

This study suggested that Epithalon might have the potential to modify the chromosome regions in the aging cells, activate chromatin, and restore cellular activities that were otherwise suppressed or delayed in geriatric subjects

What to Expect in Months 7-11 Essentially no side effects: Appetite suppression continues (desired effect), but GI symptoms gone Weight loss velocity slows: From 0.8kg/week (months 3-6) to 0.3-0.5kg/week Plateau around week 40-48: Weight stabilizes, no longer losing despite continued medication Total weight loss: Average 20-24% from baseline (matching or exceeding surgical outcomes) Focus shifts to maintenance: Accepting current weight, preventing regain Plateau frustration: Not a side effect but an emotional challenge 2 Final Strategies for Months 7-11 20