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Despite the modest body weight gain, the loss of brain tissue weight in the alcohol groups was attributed to brain shrinkage due to loss of white and gray matter, which has been documented in chronic alcohol abusers [26,27]

Rest periods between exercises were 1-2 min

VPS, ventriculoperitoneal shunt

Subsequently, the GC cells were fixed (4% paraformaldehyde) for 5 minutes, permeated (0.1% Triton X-100) for 20 minutes, and blocked (5% bovine serum albumin (BSA)) for 1 hour