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Traditional sterilization methods such as steam, gamma radiation, and ethylene oxide (EtO) introduce conditions that can compromise these molecules: Heat can disrupt secondary and tertiary structures Radiation can cause fragmentation or cross-linking Reactive gases may alter functional groups or induce oxidative stress While these approaches remain effective for many materials, they often create trade-offs between sterility assurance and molecular preservation

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The addition of other compounds does not change the subcutaneous targeting or the risks associated with accidental muscle injection