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These arrays are Human Antibody Arrays consisting of 1000 antibodies immobilised on nitrocellulose membrane ( Free BioGEE was removed from THP-1 cell lysates and supernatants by eluting from a desalting PD10 column with PBS

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To measure the concentration of 6-Fns, 10 L 6-Fns was added to 200 L XO-solution and incubated 10 min at 100 C, followed with the absorption measurement