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Description

Proteins were diluted to 25 g in Pierce RIPA Buffer lysis reagent with 1X Laemmli Sample Buffer (Bio-Rad) containing 10% -mercaptoethanol and incubated for 10 min at 70 C

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6 Impact of Opioids on the Kidneys Opioids comprise a host of illegal and prescription drugs, including heroin, fentanyl, oxycodone (OxyContin), morphine, codeine, and others

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Insulin resistance is induced through the inhibition of IRS-1 phosphorylation by TNF- (9)

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