The difference between subjects over time was also significant ( p = 0.002)
Sections were then incubated overnight at 4 C with primary antibodies, including anti-xCT (1:250, Abcam, ab307601), anti-GPX4 (1:50, Abcam, ab125066), anti-LCN2 (1:250, Abcam, ab216462), anti-MDA (1:200, Abcam, ab243066), anti-SOST (1:100, R&D Systems, BAF1589), anti-DKK1 (1:100, ProteinTech, 21112-1-AP), and anti-active--catenin (1:500, Abcam, ab305261)
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