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Mouse liver lysates were assayed on western blots (above)

This instability may result from inadequate demethylation of conserved non-coding sequence regions in the Foxp3 locus, known as the Treg-specific demethylation region (TSDR), which is fully demethylated in thymus-derived Tregs (tTregs) but often methylated in iTregs ( in vitro systems, and their physiological relevance, particularly in human disease, requires further validation

To assess the effect of PP2C30/49/51 on SAPK2-mediated ABI5 phosphorylation, PP2Cs-GST proteins were introduced into the co-expression system

10.1038/s41582-019-0217-x 72 WuY.WeiH.LiP.ZhaoH.LiR.YangF