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Additional wells were used for: LDH background (serum-free medium), maximum LDH activity control (10 lysis buffer) and spontaneous LDH activity control (water)

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As a result, recent strategies have been shifted toward developing non-invasive drug delivery methods to improve brain drug delivery, including the use of prodrugs, nanoparticles, focused ultrasound, and intranasal (IN) delivery methods (Terstappen et al., 2021)