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A limitation of our study is that we did not confirm the levels of these two metabolites in the blood plasma of clinical HCC patients

They are produced in bone marrow from hematopoietic stem cells and typically circulate for a much shorter period than red blood cells from less than a day to a few weeks (Pillay 2010

ZIP7 resides on the ER membrane and releases zinc into the cytosol (following phosphorylation by CK2), thereby acting as a second messenger to activate signaling cascades such as AKT and ERK

The same cell health tools you rely on in 2D are validated for 3D so your protocols, your data, and your confidence carry over