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Then cells were incubated at 37C for 14 h in a humidified, 5% CO 2 atmosphere
Mitochondrial respiratory complex activity measurement The enzymatic activities of complex I (NADH ubiquinone reductase), complex II (succinate ubiquinone reductase), complex III (ubiquinol cytochrome C reductase), and complex IV (cytochrome C oxidase) were measured in macrophages using enzyme activity assay kits (A089-1-A089-4, Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer's instructions
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