Particular targets appeared for the beta-blockers, carbonic anhydrase inhibitors, alpha-adrenergic agonists (aqueous humor inflow inhibition) [35,36,37,38,39], muscarinic agonists, and rho kinase inhibitors (aqueous humor outflow stimulation) [36,37,38,39]
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It's very clear that a lot of them are contaminated with things that both in the short term, but especially in the long term can be problematic
Researchers raise doses in animals until roughly 50% die, giving a crude measure of acute lethality that does not translate directly to human safety but gives context for risk margins