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This was performed using a coupled online system consisting of both the Bruker trapped ion mobility time of flight mass spectrometer (Bruker Scientific, Billerica, MA), and the Evosep One liquid chromatography platform (Evosep, Odense, Denmark)

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The additional N-terminal sequence and amino acid substitution are believed to support its biological activity by potentially increasing its affinity for IGF receptors while reducing its binding to proteins that may deactivate it (1)