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200 L aliquots were mixed with 400 L of a saline buffer solution (50 mM sodium phosphate buffer in D 2 O containing 0.05% of the internal standard TSP, trimethylsilyl) propionate-2,2,3,3,- d4 , for chemical shift calibration, and 4.5 mM NaN 3 , at a pH 7.4) to minimize the variation in the pH

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On the other hand, the pathogenesis of AAV involves many immunological processes and responses, and particularly, the role of reactive oxygen species (ROS) is known to contribute significantly to vascular inflammation and damage (Kitching et al., 2020