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doi: 10.1136/rmdopen-2014-000012
Sub-culturing was done with pre-warmed 1X PBS and 0.25% trypsin (both Thermo Scientific)
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In addition, presence of high concentrations of polyunsaturated fatty acids in the brain enhances their peroxidation and generation of toxic products which further causes degeneration of the cells harboring them or in vicinity 22
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