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Neurotransmitter agonists/antagonists, including glutamate (Sigma, G3291, USA), NMDA receptor antagonist, 2-amino-5-phosphonovaleric acid (APV) (Sigma, A8054, USA), AMPA/kainate receptor antagonist 6-Cyano-7-nitroquinoxaline-2, 3-dione (CNQX) (Sigma, C127, USA), GABA (Sigma, A2129, USA), GABA-A receptor antagonist and Bicuculline (Bic) (Sigma, 285269, USA), were dissolved in CSF tracer before use at a concentration of 0.2 mM

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