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Effectiveness and safety of Bifidobacterium and berberine in human hyperglycemia and their regulatory effect on the gut microbiota: a multi-center, double-blind, randomized, parallel-controlled study

2.2 DNA extraction Fecal samples were defrosted and resuspended in 10 mL of phosphate-buffered saline (PBS [pH 7.2]) (containing, per liter, 8 g of NaCl, 0.2 g of KCl, 1.44 g of Na 2 HPO 4 , and 0.24 g of KH 2 PO 4 , all from Sigma-Aldrich) and then centrifuged first at 2,000 rpm at 4 C for 5 min to remove fecal debris and then at 13,000 rpm for 2 min in an Eppendorf 5810R centrifuge to pellet bacterial cells

Ferroptosis inhibitors Ferroptosis is an iron-catalyzed, non-apoptotic form of regulated necrosis that causes oxidative lipid damage in cell membranes leading to m -Dys 498

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