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GHS-R1a expression in the liver has been confirmed at mRNA and protein levels, though at lower abundance than in hypothalamic and pituitary tissues
Pancreatic function under conditions of fasting and physical exercise The role of the pancreas in the present experiment can be concluded based on changes in the amylase activity, which was significantly reduced as a result of 8 days of water-only fasting
Histopathological examination To obtain paraffin-embedded tissue sections, formalin-fixed lung tissue specimens were drained using escalating grades of ethanol, cleansed with xylene, impregnated in paraffin wax, and sectioned at 4.5 m
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