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Assist in the development of coping skills and techniques to aid in the prevention of hair-pulling: Motivate your loved one to try pressing and squeezing a stress ball, pulling single threads from a piece of cloth, or fidgeting with a fidget toy as an example of a substitute habit that still offers sensory stimulation

For Ube2d3-His 6 , the gene was cloned into pGEX-4T1 for expression as an N-terminal GST fusion with a TEV cleavage site