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At least 30 microglia per field were analyzed in 3 sections/4 slides per animal

Images were captured with a CoolSNAP MYO camera (Photometrics, Tucson, AZ, USA) mounted on the microscope, and acquisition was controlled using SlideBook 5.0 software (3i Intelligent Imaging Innovations, Denver, CO, USA), which managed time-lapse acquisition, z-stack scanning, and camera triggering

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