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Identifying the functional contribution of the defatty-acylase activity of SIRT6

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CB2 receptor activation ameliorates the proinflammatory activity in acute lung injury induced by paraquat

An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)