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In general, single-end sequencing is sufficient in case of well-annotated organisms and frequently focuses only on mRNAs, while pair-end sequencing is recommended for de novo transcript discovery or isoform analysis

That is not a small distinction

Other than that, low vitamin B12 levels are more common in those who are: Older than 65 years of age

just a quick comment - trigeminal neuralgia can be a symptom of B12 deficency...and if you have B12 deficeny you may also develop other deficiencies...prime examples are iron, vitamin D and folate (folate and B12 work together so if folate is low, the body can't absorb B12 properly)